How to calculate the conversion factor for a diagnostic qPCR assay against Hepatitis B virus designed in-house?

26 July 2022 0 8K Report

Hello everyone! I have designed a diagnostic qPCR assay against hepatitis B virus with quantitative standards calibrated against WHO international standards. I have designed the viral load detection in terms of IU/ml and I want to know how to calculate the conversion factor to be given for the conversion of viral load from IU/ml to copies/ml? Thank you in advance.

Badges
Science topic

More Ravi Tauran's questions See All

Calculate the Strain at a Node in the Turbine Blade FE Model?

Hello Team, I would like to calculate the strain at a node #5 in a direction (l1,m1,n1) in the turbine blade FE model as shown. This node is connected with four elelemnts (#1,#2,#3,#4) and I have...

11 January 2021 7,702 3 View

One invention granted for the US and European patent will be counted as one patent or 2 patents for CV writing?

05 January 2021 8,135 4 View

Buckling of Smart composite plates using FEM ( MATLAB code)?

Can anyone help me with the FEM coding for Buckling of composite plates using the FSDT or HSDT - Source or the helping material (textbook, research paper etc) - Geometric stiffness matrix...

03 January 2021 3,059 3 View

How to optimize a structure for excited state when there is a bond breaking during optimization in gaussian?

In Gausssian, I have tried to reoptimize that structure by freezing the bond which is breaking in excited state during optimization. Before freezing the bond i have already tried scf = xqc and scf...

20 December 2020 8,444 3 View

How to build a polymer pdb file from a monomer pdb file, for using it is an initial configuration in Gromacs?

I am doing my Ph.D. in Materials Science and am completely new to Molecular Dynamics Simulation and Linux. I am using Manjaro Linux 20.1 and have Gromacs 2020.4, VMD 1.9.3, packmol 20.0.10,...

28 October 2020 1,051 3 View

Need help in designing a countour which create a seperation bubble on a flat plate ?

Hi This is Ravi. i am trying to create a separation bubble on a flat plate using a contoured upper wall. I want to apply same pressure distribution of a given blade on the flat plate. I have read...

21 October 2020 6,635 5 View

Can someone suggest to me Fluorescence camera for intravital microscopy of blood vessels?

Can someone suggest to me a good and not so expensive Fluorescence camera for intravital microscopy of blood vessels? or can someone advise me on choosing the right camera for intravital...

15 October 2020 4,461 1 View

Moment curvature plot in SAP2000: What should i refer to understand the background calculation which SAP2000 does to generate the M-phi ?

The moment-curvature plot in SAP2000 is based on the material stress-strain plot of caltran (along with its material FoS). I would like to understand the detailed calculation to obtain the plot...

13 August 2020 5,729 3 View

I am requesting you all to assist in the direction of epidemic diseases outbreak prediction using machine learning or deep learning approaches?

Any one who are in that track kindly give an idea of how to forward in this idea. Thanking you all

26 July 2020 2,898 3 View

How to extract electron and hole wave functions of a quantum well in Crosslight APSYS?

I am working on III Nitride multi-quantum well light-emitting diodes using Crosslight APSYS. Here I would like to extract the electron and hole wave functions, Can anyone please suggest how to...

19 June 2020 8,097 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View