I am preparing a radiolabeled product using a radiolabeled substrate. I have the cpm values for different dilutions of both my known substrate and unknown product ? How can one determine the conversion factor for a radiolabeled substrate to a radiolabeled product using slopes from linear regression equation?
Values of cpm depend only on the amount of radioactive isotope. The bear no information as to what compound the isotope is attached to. To distinguish the product from the starting material one should analyze the reaction mixture by spectroscopic or chromatographic methods. The choice of analytical method depends on the structures of the starting material and the product. Prior to carrying out the radiolabeling, one should work out the analysis using the cold materials.
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