Ravi Tauran

4 Questions 5 Answers 0 Followers

Questions related from Ravi Tauran

How to calculate precision for an assay with panel members of different viral load?

A quantitative RT PCR assay was performed with a panel of low, weak, medium and high positive and negative members in one batch for 5 days at 3 different (sites, machines and operator). How to...

07 August 2023 9,063 0 View

QPCR curves dropping - foxtails arising in first few cycles. How to avoid it?

Hello all, I am preparing a diagnostic kit for the detection of viral nucleic acid and testing them using Taqman probes. I am getting initial rise of the curves by around 1000 RFU and then...

11 May 2023 4,722 2 View

How to calculate the conversion factor for a diagnostic qPCR assay against Hepatitis B virus designed in-house?

Hello everyone! I have designed a diagnostic qPCR assay against hepatitis B virus with quantitative standards calibrated against WHO international standards. I have designed the viral load...

26 July 2022 7,905 0 View

How to calculate the HBV DNA copy numbers from quantitative standards?

I am testing HBV DNA (unknown copy number) along with HBV quantitative controls 1, 2, 3 and 4, with values 2e7, 2e6, 2e5 and 2e4 IU/ml respectively, using 3 different primer-probe concentrations...

23 May 2022 8,958 14 View