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Questions related from Ravi Tauran
Dear all, I am working on a competitive ELISA against a biochemical parameter for which I use streptavidin-coated plates, commercial Mab (biotinylated inhouse) and Mab-HRP. My sample type is...
07 August 2025 1,142 1 View
I am studying precision for my assay/kit with the following parameters followed: Panel members to be tested: 1. Weak positive 2. Medium positive 3. Strong positive 4. Negative All these were...
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A quantitative RT PCR assay was performed with a panel of low, weak, medium and high positive and negative members in one batch for 5 days at 3 different (sites, machines and operator). How to...
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Hello all, I am preparing a diagnostic kit for the detection of viral nucleic acid and testing them using Taqman probes. I am getting initial rise of the curves by around 1000 RFU and then...
11 May 2023 4,792 2 View
Dear All, I am working on quantitative real-time PCR assays in the detection of viruses. In that is it better to have the quantitative standard dilutions of higher concentrations ranges or to have...
15 February 2023 8,478 4 View
Hello, In qPCR having a set of primers and a Taqman probe, how to determine the size of the amplicon (to be) without running a gel from the PCR product? Would the presence/position of a hydrolysis...
10 October 2022 3,812 2 View
Hello everyone! I have designed a diagnostic qPCR assay against hepatitis B virus with quantitative standards calibrated against WHO international standards. I have designed the viral load...
26 July 2022 7,952 0 View
I am testing HBV DNA (unknown copy number) along with HBV quantitative controls 1, 2, 3 and 4, with values 2e7, 2e6, 2e5 and 2e4 IU/ml respectively, using 3 different primer-probe concentrations...
23 May 2022 9,045 14 View
