Hello
I am currently engaged in research focusing on plant genetic transformation. As part of this endeavor, I have designed a comprehensive in silico plasmid cloning approach. The aim is to enhance the accuracy and efficiency of our genetic engineering efforts in plants.
I welcome and deeply value input and suggestions from all stakeholders involved in this research. Your insights and expertise are crucial as we strive to optimize our methodologies and achieve impactful results in the field of plant genetic transformation.
Together, through collaboration and exchange of ideas, we can ensure the effectiveness and precision of our approaches, ultimately advancing our understanding and application of plant genetic engineering for various beneficial purposes.
Anyone is invited to write me at [email protected]
Thanks in advance everybody
If your question is whether this plasmid will work well in plants, the answer is that it will not. The above plasmid is designed as an E. coli expression vector and only for certain strains of E. coli. The plasmid will not replicate in plants nor will the promoters function unless you also provide the T7 RNA polymerase.
Yes, you are right. I am planning to work in plant. This has been designed as a cloning vector. My aim is to construct a plant binary vector. So as a cloning vector is this ok?
Could you please give your email or WhatApp number so that can take your advice directly?
It looks like a fine cloning vector. The binary vector will need left and right borders or it won't be stably integrated into the genome (if that is a goal).
As a quick note, the His tag may prove problematic if you want to use it for Western blotting. Some species of plants (Arabidopsis is one) show a very high background level of signal for anti-His.
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