Can you help me explain these RNAi results?

08 September 2014 6,023 0 View

Can anyone suggest where I can find the complete sequence of HeLa cells ?

As HeLa cells are derived from human cervical cancer cells, does this mean that the genome sequence of Hela cells will be almost similar (means ~ 99%) to human normal human cells (human genome...

02 March 2014 1,872 4 View

What are the software/online tools for miRNA:miRNA and miRNA:mRNA interaction network?

I did deep sequencing and now interested to make a network between miRNAs influencing other miRNAs/mRNA (genes) belonging to different pathways and GOs (gene ontologies). Can anybody suggest any...

11 December 2013 3,291 11 View

If the Star read count and loop read count of a novel miRNA is zero but has some mature read count, can we accept it?

In my deep sequencing data I have some microRNAs with zero star and loop read count but has some mature read count with significant p-value. Can I say that it's a true novel miRNA and should be...

10 November 2013 4,485 4 View

Can we include non-significant data while making a heat-map?

I did deep sequencing for microRNAs using 8 different kind of treatments. Now I am making the heat -map for deferentially expressed microRNAs. Most of the microRNAs having good differential...

09 October 2013 333 6 View

What do these values mean ( -1.79769e+308 or 1.79769e+308 ) in terms of differential expression (log2 fold change)?

While checking for deferentially expressed miRNAs after deep sequencing, i got some strange values like 1.79769e+308 or 1.79769e+308, which i can't understood. The rest of the values are +1, +2,...

09 October 2013 9,247 10 View

Does anyone have an expertise in statistical software "R"?

I am using R software to make heat map for differential expression of microRNAs from deep sequencing data. Following this command e.g. "nba_heatmap

08 September 2013 3,940 2 View

Is there any database for checking the expression of different genes in specific organs/cells?

I am working with miRNAs and their respective target genes in mice but i am not sure whether the respective target genes are expressing in the respective organs/cells or not ?

05 June 2013 5,164 2 View

What is best way to find a precise target for miRNA?

By using different target prediction softwares I am getting lot of targets for a single miRNA which further vary on the basis of software used. So how to finalize a specific target to further...

31 December 2012 7,818 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View