Can you help me explain these RNAi results?

08 September 2014 6,023 0 View

What are the software/online tools for miRNA:miRNA and miRNA:mRNA interaction network?

I did deep sequencing and now interested to make a network between miRNAs influencing other miRNAs/mRNA (genes) belonging to different pathways and GOs (gene ontologies). Can anybody suggest any...

11 December 2013 3,291 11 View

If the Star read count and loop read count of a novel miRNA is zero but has some mature read count, can we accept it?

In my deep sequencing data I have some microRNAs with zero star and loop read count but has some mature read count with significant p-value. Can I say that it's a true novel miRNA and should be...

10 November 2013 4,485 4 View

Can we include non-significant data while making a heat-map?

I did deep sequencing for microRNAs using 8 different kind of treatments. Now I am making the heat -map for deferentially expressed microRNAs. Most of the microRNAs having good differential...

09 October 2013 333 6 View

What do these values mean ( -1.79769e+308 or 1.79769e+308 ) in terms of differential expression (log2 fold change)?

While checking for deferentially expressed miRNAs after deep sequencing, i got some strange values like 1.79769e+308 or 1.79769e+308, which i can't understood. The rest of the values are +1, +2,...

09 October 2013 9,247 10 View

Does anyone have an expertise in statistical software "R"?

I am using R software to make heat map for differential expression of microRNAs from deep sequencing data. Following this command e.g. "nba_heatmap

08 September 2013 3,940 2 View

Is there any database for checking the expression of different genes in specific organs/cells?

I am working with miRNAs and their respective target genes in mice but i am not sure whether the respective target genes are expressing in the respective organs/cells or not ?

05 June 2013 5,164 2 View

What is best way to find a precise target for miRNA?

By using different target prediction softwares I am getting lot of targets for a single miRNA which further vary on the basis of software used. So how to finalize a specific target to further...

31 December 2012 7,818 2 View

How to analyse deep sequencing data?

From where I can get the complete information regarding next generation sequence/deep sequencing data analysis ?

07 August 2012 8,410 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View