Can you help me explain these RNAi results?

08 September 2014 6,023 0 View

Can anyone suggest where I can find the complete sequence of HeLa cells ?

As HeLa cells are derived from human cervical cancer cells, does this mean that the genome sequence of Hela cells will be almost similar (means ~ 99%) to human normal human cells (human genome...

02 March 2014 1,872 4 View

What are the software/online tools for miRNA:miRNA and miRNA:mRNA interaction network?

I did deep sequencing and now interested to make a network between miRNAs influencing other miRNAs/mRNA (genes) belonging to different pathways and GOs (gene ontologies). Can anybody suggest any...

11 December 2013 3,291 11 View

If the Star read count and loop read count of a novel miRNA is zero but has some mature read count, can we accept it?

In my deep sequencing data I have some microRNAs with zero star and loop read count but has some mature read count with significant p-value. Can I say that it's a true novel miRNA and should be...

10 November 2013 4,485 4 View

Can we include non-significant data while making a heat-map?

I did deep sequencing for microRNAs using 8 different kind of treatments. Now I am making the heat -map for deferentially expressed microRNAs. Most of the microRNAs having good differential...

09 October 2013 333 6 View

What do these values mean ( -1.79769e+308 or 1.79769e+308 ) in terms of differential expression (log2 fold change)?

While checking for deferentially expressed miRNAs after deep sequencing, i got some strange values like 1.79769e+308 or 1.79769e+308, which i can't understood. The rest of the values are +1, +2,...

09 October 2013 9,247 10 View

Is there any database for checking the expression of different genes in specific organs/cells?

I am working with miRNAs and their respective target genes in mice but i am not sure whether the respective target genes are expressing in the respective organs/cells or not ?

05 June 2013 5,164 2 View

What is best way to find a precise target for miRNA?

By using different target prediction softwares I am getting lot of targets for a single miRNA which further vary on the basis of software used. So how to finalize a specific target to further...

31 December 2012 7,818 2 View

How to analyse deep sequencing data?

From where I can get the complete information regarding next generation sequence/deep sequencing data analysis ?

07 August 2012 8,410 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View