I am using R software to make heat map for differential expression of microRNAs from deep sequencing data. Following this command e.g. "nba_heatmap
Try using heatmap.2() from the "gplots" package library instead.
The book, “interactive and dynamic graphics for data analysis with R and GGobi “ by D. Cook and D. Swayne provides the tools to create all kind of plots using an open source that you can download from http://www ggobi.org
We used RNAi to partially silence inhibin and check the effects of this silencing on some genes by qPCR like ERβ, CYP19, CYP11A1, AR, 3βHSD, IGF-I, Kitl, AMH, LHβ and FSHβ . The results indicated...
08 September 2014 6,109 0 View
As HeLa cells are derived from human cervical cancer cells, does this mean that the genome sequence of Hela cells will be almost similar (means ~ 99%) to human normal human cells (human genome...
02 March 2014 1,973 4 View
I did deep sequencing and now interested to make a network between miRNAs influencing other miRNAs/mRNA (genes) belonging to different pathways and GOs (gene ontologies). Can anybody suggest any...
11 December 2013 3,378 11 View
In my deep sequencing data I have some microRNAs with zero star and loop read count but has some mature read count with significant p-value. Can I say that it's a true novel miRNA and should be...
10 November 2013 4,559 4 View
I did deep sequencing for microRNAs using 8 different kind of treatments. Now I am making the heat -map for deferentially expressed microRNAs. Most of the microRNAs having good differential...
09 October 2013 414 6 View
While checking for deferentially expressed miRNAs after deep sequencing, i got some strange values like 1.79769e+308 or 1.79769e+308, which i can't understood. The rest of the values are +1, +2,...
09 October 2013 9,368 10 View
I am working with miRNAs and their respective target genes in mice but i am not sure whether the respective target genes are expressing in the respective organs/cells or not ?
05 June 2013 5,246 2 View
By using different target prediction softwares I am getting lot of targets for a single miRNA which further vary on the basis of software used. So how to finalize a specific target to further...
31 December 2012 7,901 2 View
From where I can get the complete information regarding next generation sequence/deep sequencing data analysis ?
07 August 2012 8,487 5 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View