Hamid Ali Khan

10 Questions 24 Answers 0 Followers

Questions related from Hamid Ali Khan

Can you help me explain these RNAi results?

We used RNAi to partially silence inhibin and check the effects of this silencing on some genes by qPCR like ERβ, CYP19, CYP11A1, AR, 3βHSD, IGF-I, Kitl, AMH, LHβ and FSHβ . The results indicated...

09 September 2014 6,076 0 View

Can anyone suggest where I can find the complete sequence of HeLa cells ?

As HeLa cells are derived from human cervical cancer cells, does this mean that the genome sequence of Hela cells will be almost similar (means ~ 99%) to human normal human cells (human genome...

03 March 2014 1,929 4 View

What are the software/online tools for miRNA:miRNA and miRNA:mRNA interaction network?

I did deep sequencing and now interested to make a network between miRNAs influencing other miRNAs/mRNA (genes) belonging to different pathways and GOs (gene ontologies). Can anybody suggest any...

12 December 2013 3,358 11 View

If the Star read count and loop read count of a novel miRNA is zero but has some mature read count, can we accept it?

In my deep sequencing data I have some microRNAs with zero star and loop read count but has some mature read count with significant p-value. Can I say that it's a true novel miRNA and should be...

11 November 2013 4,538 4 View

What do these values mean ( -1.79769e+308 or 1.79769e+308 ) in terms of differential expression (log2 fold change)?

While checking for deferentially expressed miRNAs after deep sequencing, i got some strange values like 1.79769e+308 or 1.79769e+308, which i can't understood. The rest of the values are +1, +2,...

10 October 2013 9,334 10 View

Can we include non-significant data while making a heat-map?

I did deep sequencing for microRNAs using 8 different kind of treatments. Now I am making the heat -map for deferentially expressed microRNAs. Most of the microRNAs having good differential...

10 October 2013 391 6 View

Does anyone have an expertise in statistical software "R"?

I am using R software to make heat map for differential expression of microRNAs from deep sequencing data. Following this command e.g. "nba_heatmap

09 September 2013 3,998 2 View

Is there any database for checking the expression of different genes in specific organs/cells?

I am working with miRNAs and their respective target genes in mice but i am not sure whether the respective target genes are expressing in the respective organs/cells or not ?

06 June 2013 5,218 2 View

What is best way to find a precise target for miRNA?

By using different target prediction softwares I am getting lot of targets for a single miRNA which further vary on the basis of software used. So how to finalize a specific target to further...

01 January 2013 7,873 2 View

How to analyse deep sequencing data?

From where I can get the complete information regarding next generation sequence/deep sequencing data analysis ?

08 August 2012 8,459 5 View