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Questions related from Hamid Ali Khan
We used RNAi to partially silence inhibin and check the effects of this silencing on some genes by qPCR like ERβ, CYP19, CYP11A1, AR, 3βHSD, IGF-I, Kitl, AMH, LHβ and FSHβ . The results indicated...
09 September 2014 6,079 0 View
As HeLa cells are derived from human cervical cancer cells, does this mean that the genome sequence of Hela cells will be almost similar (means ~ 99%) to human normal human cells (human genome...
03 March 2014 1,930 4 View
I did deep sequencing and now interested to make a network between miRNAs influencing other miRNAs/mRNA (genes) belonging to different pathways and GOs (gene ontologies). Can anybody suggest any...
12 December 2013 3,360 11 View
In my deep sequencing data I have some microRNAs with zero star and loop read count but has some mature read count with significant p-value. Can I say that it's a true novel miRNA and should be...
11 November 2013 4,540 4 View
While checking for deferentially expressed miRNAs after deep sequencing, i got some strange values like 1.79769e+308 or 1.79769e+308, which i can't understood. The rest of the values are +1, +2,...
10 October 2013 9,337 10 View
I did deep sequencing for microRNAs using 8 different kind of treatments. Now I am making the heat -map for deferentially expressed microRNAs. Most of the microRNAs having good differential...
10 October 2013 393 6 View
I am using R software to make heat map for differential expression of microRNAs from deep sequencing data. Following this command e.g. "nba_heatmap
09 September 2013 4,000 2 View
I am working with miRNAs and their respective target genes in mice but i am not sure whether the respective target genes are expressing in the respective organs/cells or not ?
06 June 2013 5,220 2 View
By using different target prediction softwares I am getting lot of targets for a single miRNA which further vary on the basis of software used. So how to finalize a specific target to further...
01 January 2013 7,875 2 View
From where I can get the complete information regarding next generation sequence/deep sequencing data analysis ?
08 August 2012 8,461 5 View