Is it justified to compare the free energy change of two reactions and conclude one is more favourable than the other?

Suppose we consider the binding of a ligand L to a metal M, M+L=ML and M+2L=ML2 . We compute the Gibbs free energy change for two reaction using DFT. But can we compare this two free energies to...

12 January 2021 5,893 4 View

How can i obtain electric displacement field in Piezoelectric bonded joint using Abaqus?

Mathematically, electric displacement is a vector quantity defined as {D}=([e]{E}+{P}), where {E} is local electric field, {P} is Polarization per unit volume and [e] is the third order tensor...

20 August 2020 4,789 1 View

How to calculate van der Waals radius theoretically?

I am trying to calculate van der Waals radius for silver atoms and ions. Could anyone explain the steps required to perform accurate calculations.

03 August 2020 2,574 3 View

How to do overlap PCR?

Hi I am trying to join two linear 1.2 kb fragments with overlaps. I read a protocol which says at least 1/2 of the PCR mix has to be the equimolar concentrations of the fragment. Why is so much...

30 May 2019 9,234 2 View

Is it possible to choose a site for setting a well using only remote sensing techniques and data ?

If I have only Microwave and Optical remotely sensed data, is it possible to choose the site for availability of ground water for set a pump or well so that it will reduce the chances of fail...

05 December 2018 3,372 13 View

How to create checkpoint file in molecular dynamics simulation?

Basically I want to save coordinates and momentum of atoms after n time step so that I can restart my calculation in case of an error or exceeding wall time. My MD code is in Fortran.

19 June 2018 8,262 5 View

What if Sodium pyruvate is added in excess in DMEM cell culture media?

What happens if we accidentally add more (5 ml of 200 mM) sodium pyruvate to 500 ml DMEM cell culture media that already contains 110 mg/ml sodium pyruvate in it (from company)? How will it...

29 November 2017 6,582 2 View

What are the different approaches for seperate few layer graphene from graphite?

31 August 2017 1,378 4 View

Availability of Sugarcane yellow leaf virus antibody/antiserum from any private firm?

Dear all...is there any information regarding availability of Sugarcane yellow leaf virus antibody/antiserum. previously i use to procure from AC, Diagnostics, USA but now they have discontinued...

24 January 2017 4,333 3 View

Is h index a good metric of a researcher's credibility?

Higher h index demands larger uniformity in citation pattern. Therefore, a researcher with lesser citations may get a higher h index only due to larger uniformity in the total citations.

05 June 2016 1,360 4 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View