Somnath Chakravorty

4 Questions 4 Answers 0 Followers

Questions related from Somnath Chakravorty

Site directed mutagenesis not working. Can anyone help?

I am trying to replace a 20 bp region with a different sequence of same lengthin a 10kb plasmid. I designed overlapping primers. However after PCR I am only getting the template. At first I...

14 February 2023 3,637 3 View

THP-1 requires differentiation before Phagocytosis assay?

I am looking to study opsonophagocytosis using THP-1 cell line from ATCC. Does the cell line require differentiation before the phagocytosis assay?

29 October 2021 3,376 5 View

How to align large plasmids (20Kb+) in Mac Vector?

I was trying to align 3 plasmids each around 20Kb in Mac Vector. I used the "Align to ref." tab followed by adding the sequences and then aligned them. However only a portion of each sequence is...

04 February 2020 2,757 1 View

How to do overlap PCR?

Hi I am trying to join two linear 1.2 kb fragments with overlaps. I read a protocol which says at least 1/2 of the PCR mix has to be the equimolar concentrations of the fragment. Why is so much...

31 May 2019 9,365 2 View