Does the pre-synaptic neuron location in a cortical map influences the spatial position of its synapses in the dendrite of the downstream neuron?

Are functional maps in the cortex used by the brain to carry out computations or are they just a byproduct of wiring minimization? A key element to answer this question is to know if, when...

26 January 2021 1,118 8 View

Which are recommended ways to catch beehive information? By knowing that beehives are far away from internet connection?

I have seen around in the web different technologies focused towards beehive health. Beekeeping is a millenial activity, by saying this, beehives are located far away from where Internet...

21 October 2020 3,750 1 View

What is the best metric to measure the fragmentation of a specific landcover class in an individual homerange?

I need to extract a measure of fragmentation for each landcover class found in a individual homerange of a bird. E.g. level of fragmentation of grassland within the homerange of the bird...

13 October 2020 843 7 View

Isn't it time to reduce the working week? Working all, less and better. I can't find recent and relevant research on the topic. Can you help?

Dear Colleagues, many challenges ahead of us. The pandemic crisis and its repercussions on employment, the coming climate and ecological apocalypse, the aging population (especially in some...

14 July 2020 9,573 2 View

Covid-19 and Circular Economy. Any analysis, works, prospects to recommend?

Dear colleagues, I would like to create a repository with documents, reports and scientific articles that speak about COVID-19 and Circular Economy. The aim is to try to draw future potential...

31 May 2020 1,319 25 View

Information system for risk management as a predicting variable in a study for COVID-19. Any measurement/survey?

Dear colleagues, I am trying to find a measurement/survey with items that assess the adoption/deployment/development of an information system as a risk management tool. I would like to use it as...

19 May 2020 711 4 View

Interesting work on the relationship between Sensing, Seizing and Reconfiguring DCs?

Hello everyone. I am working on my second article for my Ph.D. thesis. The first one was a systematic literature review on the way researchers have so far measured DCs for Sustainability. Now I am...

10 February 2020 3,680 3 View

Do you know visual stimuli in which the pop-out of an object-shape can be controlled by a priming cue?

03 August 2019 7,646 6 View

Which collaboration website/software would you recommend to share files in a team project?

Evaluation criteria: - freeware - quick to learn (simplicity over advanced functions) - minimal installation requirements in term of memory (online also an option) Thank you

13 March 2019 6,736 2 View

Is it possible to build species accumulation curves with multiple surveys and incident data?

Hi, I would like to build a species accumulation curve using specaccum function in R vegan package. The entry table should be a classic community table species by sites. However, instead of sites,...

21 February 2019 3,351 10 View

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02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View