Laura Weber

6 Questions 4 Answers 0 Followers

Questions related from Laura Weber

When changing the medium for my cell culture with LPS activation, do I need to include LPS in the new medium as well?

I am using the RAW 264.7 cell line to do a drug release study. They are activated with 10 ug/mL LPS. When changing the medium after two or three days, do I need to add LPS to the new medium as...

30 November 2017 4,267 2 View

How stable is my primary antibody in 10% normal goat serum?

I prepared, separately, rabbit anti-rat P4HB (1:50 dilution) and rabbit anti-rat CD68 (1:100 dilution) primary antibodies in 10% normal goat serum (in TTBS w/ 0.05% Triton-X 100, as per our usual...

24 October 2017 1,212 4 View

How can I keep rat knee joint tissues intact for paraffin embedding?

I have some samples ready to be processed for paraffin embedding, but when I cut the decalcified joints into 3mm thickness sections to fit into the paraffin cassettes, the tissues tend to separate...

05 July 2017 8,553 9 View

Is it okay to transfer tissue samples from 10% EDTA in H2O to 10% EDTA in PBS?

I have been preparing 10% EDTA solution for decalcification of rat knee joints that I want to use for IHC, and have been using it for 2.5 weeks. However, even though the pH may only change by 0.2...

30 June 2017 7,355 1 View

Can I decalcify and paraffin-embed tissues that have been sucrose dehydrated and flash frozen?

I already put into sucrose gradient and flash froze several rat knee joints for IHC. I realize it may be too difficult to cut these into sections, however. Is it too late to decalcify these?

05 June 2017 8,844 3 View

How do I section/stain rat knee joints for IHC using markers for the nerves?

This may seem like a simple question, but I have never done any form of histology. I have rats with adjuvant-induced arthritis in the left knee joint, and I wish to see the fluorescence from a...

31 May 2017 2,735 14 View