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Questions related from Laura Weber
I am using the RAW 264.7 cell line to do a drug release study. They are activated with 10 ug/mL LPS. When changing the medium after two or three days, do I need to add LPS to the new medium as...
30 November 2017 4,277 2 View
I prepared, separately, rabbit anti-rat P4HB (1:50 dilution) and rabbit anti-rat CD68 (1:100 dilution) primary antibodies in 10% normal goat serum (in TTBS w/ 0.05% Triton-X 100, as per our usual...
24 October 2017 1,228 4 View
I have some samples ready to be processed for paraffin embedding, but when I cut the decalcified joints into 3mm thickness sections to fit into the paraffin cassettes, the tissues tend to separate...
05 July 2017 8,578 9 View
I have been preparing 10% EDTA solution for decalcification of rat knee joints that I want to use for IHC, and have been using it for 2.5 weeks. However, even though the pH may only change by 0.2...
30 June 2017 7,365 1 View
I already put into sucrose gradient and flash froze several rat knee joints for IHC. I realize it may be too difficult to cut these into sections, however. Is it too late to decalcify these?
05 June 2017 8,849 3 View
This may seem like a simple question, but I have never done any form of histology. I have rats with adjuvant-induced arthritis in the left knee joint, and I wish to see the fluorescence from a...
31 May 2017 2,769 14 View
I want to formulate the liposomes in ~10 mM phosphates, but I want to run some experiments in ~100 mM phosphates. Will this cause leakage of my entrapped protein and/or loss of liposome structure?
17 November 2016 551 4 View
