I'm trying to compare the three methods that have TCID50 values, but seemingly different units to make sure I understand their performance. The following reflects measurements using heat inactivated respiratory virus to determine the lower limit of detection of the method:
Method A: 340 per ml
Method B: 140 per ml
Method C: 22.5 per swab
In method C, I see a description showing that a contrived nasal swab sample was prepared by absorbing 20 microliters of each virus dilution onto the swab. Could someone clarify how to account for this? Is the TCID50 1,125 per ml then for method C?
If all the 20microlitres (.02ml) is active then i would say yes.
22.5 per swab = 22.5/20microl = 22.5/.02 ml = 1125/ml.
Thanks, James. I was thinking the same, but there's no information on the dilution value for the 20 microliters (they used a stock, did dilutions, then added 20 microliter aliquotes to make contrived samples on the swab).
I guess I assume the dilution is accounted for in the value for 'per swab' then?
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