How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

How to analyze sodium lauryl sulfate using HPLC-RID?

Hi, I am analyzing the content of anionic surfactant: sodium lauryl sulfate (SLS) in commercial detergents using HPLC-RID/refractive index detector. I used column Agilent Zorbax Eclipse XDB C-18...

15 November 2020 1,405 2 View

Seeds of wild Brassica rapa?

Hi, We are a team working on wild and cultivated plants and our stock miss of seeds of Brassica rapa from wild populations in Europe. We are based in Finland, we wonder if any laboratories in...

25 October 2020 857 3 View

Which proteins are continuously expressed (at stable levels) in mammalian monocytes and thus, suitable controls for Protein expression studies?

Hello! Currently I am testing whether an externally added agent affects the expression of only one particular protein or whether it generally inhibits protein synthesis due to its toxicity. For...

14 September 2020 6,359 3 View

Suggestions for an easy experiment on an inverted fluorescence microscope (students lab course)?

09 September 2020 290 3 View

How to do a repeated measures ANOVA with two within factors, where one factor is random and the other fixed?

We performed an experiment on a number of blood donors. We took the blood from each donor and divided it into 9 equal volumes. Three of the blood samples from each individual were left as...

17 May 2020 9,326 5 View

Why it is that Disulfiram (DSF) only binds to zinc in cancer cells?

Good Afternoon, I understand that the zinc transporter proteins (ZIP7 and ZIP10) are both present in healthy and diseased cells. I learnt that zinc uptake is elevated in malignant cells...

09 October 2019 5,069 3 View

Hypothesis test for conditional mean?

I have a single time series which I have computed a mean for. I am interested in comparing this time mean against one computed from the same time series, but with a few of the most extreme values...

13 September 2019 9,736 9 View

How to analyze alkyl distribution of Methyl Ester Sulfonate / Sulfonated Methyl Ester?

I need to analyze the alkyl distribution of Methyl Ester Sulfonate to know the molecular weight of my sample. I tried to use gas chromatography to do this. My method was to reflux the sample first...

14 July 2019 2,133 4 View

Compare multiple means over time, which analysis?

I have a repeated measures design, looking at how sex hormones effect number of responses on a dichotic listening task, three times over the course of the menstrual cycle. Where it simply the...

14 April 2019 8,108 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View