I need to check the composition of media and their identifications.
Hello everyone, I am modelling a B+G+5 building. I was getting everything correct. The pre and post analysis checks all were correct. But due to some new revisions . Now after applying post...
09 September 2022 149 0 View
I have manual but unable to get answer of some Childish Question like how to extract signal from GTAO How to connect various component. Interfacing of RTDS to Relay
04 October 2021 3,040 2 View
almost structure is same but how and which manner I say it is better than Microgrid
04 October 2021 2,554 4 View
The proceudre for estimation of Si in rice straw ash by ICP-OES is different from dry straw or the same? If different then what is the procedure for estimation of straw ash?
21 June 2021 3,145 3 View
Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...
16 February 2021 1,816 2 View
How the lift and weight cancel each other perfectly for a helicopter to stay it motionless? Because its propeller do generate huge amount of lift due to air pressure plus a blend of air stream...
09 February 2021 4,442 14 View
I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...
03 February 2021 9,608 3 View
I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...
02 February 2021 9,127 2 View
I have prepared gold nanoparticles using the citrate reduction method in the lab. I need to characterize them using UV-Vis and DLS, by how much should I dilute my sample for using it in the...
26 January 2021 744 5 View
I need to conjugate thiolated oligos to GNPs and I am wondering what concentrations of the oligo to use with what concentration of GNPs for effective conjugation.
08 December 2020 8,162 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
How Social Media Affects Your Mental Health
04 August 2024 6,961 3 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
