Solid Phase Extraction of drug product containing nanoparticles ?

Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...

16 February 2021 1,389 2 View

Can an airplane stay up in the the air without moving forward just like helicopter ?

How the lift and weight cancel each other perfectly for a helicopter to stay it motionless? Because its propeller do generate huge amount of lift due to air pressure plus a blend of air stream...

09 February 2021 3,990 14 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

What dilution do I use for my GNP solution UV-Vis and DLS characterization?

I have prepared gold nanoparticles using the citrate reduction method in the lab. I need to characterize them using UV-Vis and DLS, by how much should I dilute my sample for using it in the...

26 January 2021 530 5 View

What methods can I use to confirm if my oligo-GNP conjugate is hybridizing with my target nucleic acid?

Once I have oligo-GNP conjugates, I need to use them as sensor for my target nucleic acid. Before doing that I need to confirm whether the oligo probe (conjugated to GNP) hybridizes well with my...

04 December 2020 4,734 2 View

I need to design primers for hybridization chain reaction for signal amplification (in-vitro). Anybody know a software/company that does this?

Hybridization chain reaction (HCR), a form of non-enzymatic method for amplification of target nucleic acids. I need to design the two HCR primers. Is there any special software for doing that?

27 August 2020 1,008 1 View

Suggestion for buying speed vaccum table top for conentrating samples ?

I need a suggestion for buying a speed vac system for drying solvents/concentrating samples after SPE.

24 August 2020 648 2 View

Can we design aptamers that target other nucleic acids?

Aptamers in most cases are themselves nucleic acids, generally used to target other bio-molecules. I wonder if we can design aptamers in such a way that they target other nucleic acids. Will...

06 June 2020 3,132 1 View

How to model PV array under partial shading without bypass diode?

I have been using the photovoltaic array under partial shading in matlab/simulink. However, when i try to get the characteristics without bypass diode the simulation do not execute.

01 January 2019 7,003 6 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View