Sanat Myti

7 Questions 6 Answers 0 Followers

Questions related from Sanat Myti

What is the dielectric constant of DMEM with 10% FBS?

I want to perform nanoparticle volume and number distribution measurement where dielectric constant parameter is required. Could anyone please help me by sharing information about this value? Thanks

16 August 2018 4,930 1 View

Could anyone please help me to give an answer about C-terminal amino acid deletion during protein processing in e. coli?

I am trying to express a protein containing 213 amino acids in e.coli cells. After processing protein will be in periplasmic space. Here we use pelB as a leader sequence. Protein is producing....

17 April 2018 2,258 5 View

How should I analyze culture media using in mammalian cell culture through ESI-LC-MS/MS system?

I need to check the composition of media and their identifications.

09 March 2018 1,657 0 View

Is there any proven data about methionine (Start codon) expel during or after protein synthesis in E. Coli?

I've been facing a problem LC-MS/MS analysis. I have a protein that in produced in E. coli and it is actually deposited in inclusion body. Protein contain methionine as a start codon, during...

12 August 2017 8,578 1 View

Why threshold value set in exponential phase, if it set in linear phase for Real time PCR any problem?

10 July 2017 279 1 View

What is the maximus molecular radius ( nm scale) of biotherapeutic drug?

I am searching what is the maximum molecular radius for protein based drug and/ antibody related drug. If any one know please let me inform. Thanks in advance.

02 July 2017 6,043 3 View

Nucleic Acid Quantification??

Hello, I am facing one problem in case of quantification of nucleic acid conc. wit udrop plate which pathlength is 0.5 mm. Whether it is necessary for me to quantify the concentration in respect...

25 January 2017 1,311 3 View