Is it possible to elute IgGs bound to Protein G ligands using temperature and heating (without denaturing IgGs)?

I have modified an affinity chromatography column coated with Protein G to efficiently separate IgGs. Is it possible to elute the bound IgGs by adjusting temperature instead of utilizing elution...

09 October 2019 3,190 2 View

What are the best softwares to simulate Microfluidic Affinity Chromatography process?

I want to simulate my microfluidic design which utilizes affinity chromatography at micro-scale. I have already worked with software such as Comsol Multiphysics. Is there any other software? Which...

09 October 2019 1,907 1 View

How can I buy/order custom micro sized columns for affinity chromatography?

I want to buy chromatography columns for the affinity-based separation of proteins. The size (diameter) of the column needs to be very small (less than 100um) in order to match the design. Are...

09 October 2019 5,519 5 View

How to find out the type and name of materials by using FTIR analysis?

I have utilized FTIR (Fourier Transform Infrared Spectroscopy) to analyze and detect the material of my sample. The possible answers are obtained and I don't know which type of material they are!...

06 July 2019 4,789 16 View

What is the best method to fine-cut a plexiglass (or similar material) cylinder (d=2cm, l=5mm)?

I want the cutting resolution to be less than 0.1 mm and I don't want to damage the sample.

03 April 2019 6,100 3 View

Are there any methods to detect and find the chemical formula of a polymer (carbon, hydrogen, oxygen)?

I have a polymer material and I want to find out what type it is. I want to know its chemical formula. How many Carbons, Hydrogen and Oxygen are there? I already know that Electron Microscopy...

02 March 2019 611 8 View

What are some simple gearbox designs which could multiply speed/rotation by 2?

I want to find a gear mechanism which doubles the output rotation.

06 July 2018 8,560 9 View

Is there any formula to find convection coefficient (h) for a free/natural air inside a tube (internal flow)?

I want to find the natural heat transfer convection coefficient (h) inside the intestines. I just do not know how to calculate it.

05 June 2018 7,151 3 View

What are the main materials for mechanical bistable structures?

In mechanical engineering, a bistable structure is one that has two stable mechanical shapes, particularly where they are stabilized by different curvature axes. (Source: Wikipedia) I want to know...

04 May 2018 705 5 View

Which Standard Cam Motion is suitable for the y-theta diagram below?

I want a rise-dwell-return motion in half a cycle and I don't need the cycle to repeat. Rise: 0 - pi/4 Dwell: pi/4 - 3pi/4 Return: 3pi/4 - pi for Rise & Dwell V0=Vf=0 a0=af=0 It would be great...

04 May 2018 2,629 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View