Oscar Otero Alfaro

3 Questions 61 Answers 0 Followers

Questions related from Oscar Otero Alfaro

Can I freeze at -20 C E coli suspension for posterior extraction of DNA without affect its integrity and purity?

I am using the QIAamp DNA Mini kit 250 from QiaGen for purify genomic DNA from E. coli. I have a biomass and I am looking for the best storage conditions because I am not going to perform the DNA...

08 August 2018 3,226 3 View

How do I purify IgM MAb from ascites fluid using CIMmultus QA-1 column from BIA Separations firm?

I am looking for some protocol to purify a MAb IgM isotype from ascites fluid. I have found purifying methods to isolate IgM from plasma or serum and MAbs IgM but from culture supernatant. Please,...

08 August 2015 7,331 1 View

What is the relationship between Ka and sensitivity in sandwich ELISA?

I'm trying to search for a relationship that explains a MAb with high affinity is responsible for a sandwich ELISA with high sensitivity. It uses the same mAb as capture and as conjugate.

07 July 2014 9,292 3 View