How much range minimum nucleotides primer for polymorphism gene analysis, can 10 nucleotides?
It depends on the target genome. Usualy, specific primers for DNA fragment PCR amplification ranged from 16 to 25 nucleotides. 10 nuceotides will give non-specific amplification due to low Ta. They will set on many non-target sequences in the studied genome.
It dependents on the length of PCR fragments. 16-30 nucleotides usually be ok if PCR fragments
primers about 10 nt lenght are used for RAPDs, AFLPs and you definitely don't need shorter primers, because 10 nt is enough to get just reasonable amount of fragments for polymorphism analysis, but you must be careful with the reaction conditions - a little change in protocol will give you different result as 10 nt is not specific enough.
here's a revised AFLP protocol of improved reproducibility:
https://www.beckmancoulter.com/wsrportal/bibliography?docname=a-2015a.pdf
primers are not used in RFLP analysis, RFLPs are fragments from DNA digested with restriction enzyme, no PCR involved :)
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