How much proteinase K adding when extracting DNA from hard tick?

More Khetam Qaid Mayee's questions See All

What is the relationship between ladder and voltage in gel electrophoresis ?

ladder, gel electrophoresis, pcr

31 December 2017 6,127 3 View

How does a difference in GC content in primers affect PCR?

primer

31 December 2017 7,218 0 View

How to remove DNA contamination from extracted RNA?

TRIzol extraction, DNA contamination, RNA, Extraction

11 December 2017 5,493 5 View

What is the faster journals in publishing of genotyping and midecal research and within scopus?

Genotyping, Medical journals

05 June 2017 4,513 7 View

DNA extraction from formaldehyde preserved samples?

I have Jellyfish sample preserved in 4% formaldehyde, how could i extracte DNA ?? and What is the probability of getting enough DNA con.? DNA, Jellyfish, preserved samples

04 May 2017 8,175 4 View

What is the main viral zoonotic diseases circulating in the Arab World ?

virology, zoonotic diseases, RNA, DNA

04 May 2017 7,598 7 View

What are DNA Vaccines?

DNA

03 April 2017 2,371 6 View

I need program for phylogentic tree easily using more then MEGA6 ?

phylogentic tree, MEGA6

03 April 2017 4,667 8 View

What are the differences between extracted RNA and cDNA?

RNA, cDNA

03 April 2017 1,221 1 View

Protocol for extracting DNA from colonies ?

DNA, Protocol

03 April 2017 9,494 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View