How to remove DNA contamination from extracted RNA?

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What is the relationship between ladder and voltage in gel electrophoresis ?

ladder, gel electrophoresis, pcr

31 December 2017 6,127 3 View

How does a difference in GC content in primers affect PCR?

primer

31 December 2017 7,218 0 View

What is the faster journals in publishing of genotyping and midecal research and within scopus?

Genotyping, Medical journals

05 June 2017 4,513 7 View

DNA extraction from formaldehyde preserved samples?

I have Jellyfish sample preserved in 4% formaldehyde, how could i extracte DNA ?? and What is the probability of getting enough DNA con.? DNA, Jellyfish, preserved samples

04 May 2017 8,175 4 View

How much proteinase K adding when extracting DNA from hard tick?

proteinase K, DNA, extraction, hard tick,

04 May 2017 5,335 3 View

What is the main viral zoonotic diseases circulating in the Arab World ?

virology, zoonotic diseases, RNA, DNA

04 May 2017 7,598 7 View

What are DNA Vaccines?

DNA

03 April 2017 2,371 6 View

I need program for phylogentic tree easily using more then MEGA6 ?

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03 April 2017 4,667 8 View

What are the differences between extracted RNA and cDNA?

RNA, cDNA

03 April 2017 1,221 1 View

Protocol for extracting DNA from colonies ?

DNA, Protocol

03 April 2017 9,494 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View