What is the relationship between ladder and voltage in gel electrophoresis ?

ladder, gel electrophoresis, pcr

31 December 2017 5,937 3 View

How does a difference in GC content in primers affect PCR?

primer

31 December 2017 7,095 0 View

How to remove DNA contamination from extracted RNA?

TRIzol extraction, DNA contamination, RNA, Extraction

11 December 2017 5,346 5 View

What is the faster journals in publishing of genotyping and midecal research and within scopus?

Genotyping, Medical journals

05 June 2017 4,388 7 View

DNA extraction from formaldehyde preserved samples?

04 May 2017 8,014 4 View

How much proteinase K adding when extracting DNA from hard tick?

proteinase K, DNA, extraction, hard tick,

04 May 2017 5,210 3 View

What are DNA Vaccines?

DNA

03 April 2017 2,217 6 View

I need program for phylogentic tree easily using more then MEGA6 ?

03 April 2017 4,487 8 View

What are the differences between extracted RNA and cDNA?

RNA, cDNA

03 April 2017 1,026 1 View

What is flow cytometry?

cytometery

03 April 2017 3,287 15 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View