Pick a very small portion of colony with a sterile pipette tip or a toothpick and put it into a 10 ul of sterile distilled water. Heat it at 94 degree C for 5 min followed by cooling at 4 degree. Spin for 5 min at 10000 rpm. take sup. as template for PCR. Alternatively, you can also try direct addition of part of colony to PCR mix.