primer
ladder, gel electrophoresis, pcr
31 December 2017 6,127 3 View
TRIzol extraction, DNA contamination, RNA, Extraction
11 December 2017 5,493 5 View
Genotyping, Medical journals
05 June 2017 4,513 7 View
I have Jellyfish sample preserved in 4% formaldehyde, how could i extracte DNA ?? and What is the probability of getting enough DNA con.? DNA, Jellyfish, preserved samples
04 May 2017 8,175 4 View
proteinase K, DNA, extraction, hard tick,
04 May 2017 5,335 3 View
virology, zoonotic diseases, RNA, DNA
04 May 2017 7,598 7 View
DNA
03 April 2017 2,371 6 View
phylogentic tree, MEGA6
03 April 2017 4,667 8 View
RNA, cDNA
03 April 2017 1,221 1 View
DNA, Protocol
03 April 2017 9,494 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26
04 August 2024 9,919 4 View
How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?
02 August 2024 9,644 0 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
