What is the relationship between ladder and voltage in gel electrophoresis ?

ladder, gel electrophoresis, pcr

31 December 2017 5,937 3 View

How to remove DNA contamination from extracted RNA?

TRIzol extraction, DNA contamination, RNA, Extraction

11 December 2017 5,346 5 View

What is the faster journals in publishing of genotyping and midecal research and within scopus?

Genotyping, Medical journals

05 June 2017 4,388 7 View

DNA extraction from formaldehyde preserved samples?

04 May 2017 8,014 4 View

How much proteinase K adding when extracting DNA from hard tick?

proteinase K, DNA, extraction, hard tick,

04 May 2017 5,210 3 View

What is the main viral zoonotic diseases circulating in the Arab World ?

virology, zoonotic diseases, RNA, DNA

04 May 2017 7,443 7 View

What are DNA Vaccines?

DNA

03 April 2017 2,217 6 View

I need program for phylogentic tree easily using more then MEGA6 ?

03 April 2017 4,487 8 View

What are the differences between extracted RNA and cDNA?

RNA, cDNA

03 April 2017 1,026 1 View

What is flow cytometry?

cytometery

03 April 2017 3,287 15 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View