How to insert a simply supported boundary condition without in-plane fixation in ANSYS (Transient sructural)?

Dear all, I have a question regarding imposing a boundary condition in ANSYS (Transient Structural). The problem is a slamming of a composite plate in water (FSI problem). The geometry of the...

27 October 2020 640 3 View

Gelatin nano particles, obtained by two-step desolvation, do not re-disperse in water. What is the problem?

Hello I have synthesized gelatin type A nano-particles by two-step desolvation. After 4 days of freeze-drying, the powder was kept in the freezer (-80 C). Today I tried to redisperse the...

18 February 2020 9,494 10 View

Recombinant protein production in insect cells using plasmid-based system?

Is it possible to produce a Flag-tagged catalytic domain of a murine enzyme in insect cells using a plasmid-based system? does it affect the functionality of the final enzyme?

29 November 2019 7,504 3 View

The small dimension of gelatin nano gel. what is the problem?

Hi I am trying to Synthesis gelatin (type A) nano particles by two-step desolvation method. By DLS, the dimension of gelatin particles are less than 50 nm. I am not sure what is wrong with my...

23 July 2019 964 3 View

In MTT or Alamar Blu test for cytotoxicity assay by using UV spectrophotometery, biologists use absorbance even more than 1. Is it correct?

In MTT or Alamar Blu test for cytotoxicity assay by using UV spectrophotometery, I saw many biologist researchers that use absorbance even more than 1 for their calculations. Is it correct? As I...

10 March 2019 4,442 3 View

To make flexible polyurethane foam, necessary to observe the mixing process?

20 June 2016 8,595 7 View

What is the integrin binding site for Vitronectin?

As we know vitronectin and some other proteins can bind to different types of integrin. I want to know about the binding site in Integrin which the vitronectin with a RGD-sequence can interact...

30 January 2015 5,378 5 View

How can I extract nodal values of displacement, coordinates and time of each material point at every increment of time in ABAQUS/EXPLICIT?

How can I extract " nodal values of displacement, coordinates and time of each material point at every increment of time" in ABAQUS/EXPLICIT?

30 January 2015 2,597 3 View

Treatments have obvious difference, but ANOVA doesn't show significant difference?

Hello everyone. I attempted one way ANOVA for data set of an experiment with one factor (hydrogel treatment) at 5 levels (5 concentrations). Data showed normal distribution and variance...

26 November 2014 3,450 13 View

How can I calculate the unit of enzymes?

13 November 2014 461 16 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to classify strictly upper triangular matrices by similarity?

Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

01 March 2021 2,957 8 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View