How to insert a simply supported boundary condition without in-plane fixation in ANSYS (Transient sructural)?

Dear all, I have a question regarding imposing a boundary condition in ANSYS (Transient Structural). The problem is a slamming of a composite plate in water (FSI problem). The geometry of the...

27 October 2020 640 3 View

How is solubility of CO2 or air in organic solvents such as xylene or cyclohexane?

For some reason, I need to inject a dispersion of an organic solvent and organoclay into a block of soil sample. the organic solvent probably is xylene.

29 September 2020 6,348 4 View

What is the best solvent to dissolve nanoclay (montmorillonite)?

I need to inject nano-clay to some kind of collapsible soil (silty loess) to stabilize and strengthen it. I can't use water since this soil is moisture sensitive and water may dissolve the bonding...

26 March 2020 1,675 3 View

Gelatin nano particles, obtained by two-step desolvation, do not re-disperse in water. What is the problem?

Hello I have synthesized gelatin type A nano-particles by two-step desolvation. After 4 days of freeze-drying, the powder was kept in the freezer (-80 C). Today I tried to redisperse the...

18 February 2020 9,494 10 View

How I can eliminate 50Hz parasitic magnetic field and the at same time do not affect existed DC magnetic filed?

My application is EFNMR (Earth field nuclear magnetic resonance). In this application the earth filed, which is a DC field, has the main role and should not be altered. However, all other magnetic...

10 January 2020 337 2 View

Recombinant protein production in insect cells using plasmid-based system?

Is it possible to produce a Flag-tagged catalytic domain of a murine enzyme in insect cells using a plasmid-based system? does it affect the functionality of the final enzyme?

29 November 2019 7,504 3 View

The small dimension of gelatin nano gel. what is the problem?

Hi I am trying to Synthesis gelatin (type A) nano particles by two-step desolvation method. By DLS, the dimension of gelatin particles are less than 50 nm. I am not sure what is wrong with my...

23 July 2019 964 3 View

What are the best Water-Energy-Food Nexus Indicators at watershed scale?

water-energy-food nexus at watershed scale such as water mass productivity, water consumption and etc.

24 May 2019 7,079 3 View

In MTT or Alamar Blu test for cytotoxicity assay by using UV spectrophotometery, biologists use absorbance even more than 1. Is it correct?

In MTT or Alamar Blu test for cytotoxicity assay by using UV spectrophotometery, I saw many biologist researchers that use absorbance even more than 1 for their calculations. Is it correct? As I...

10 March 2019 4,442 3 View

How can I simulate rate of spontaneous emission of Silicon in Matlab?

I need best references of analytical solution of rate of spontaneous emission of indirect semiconductors like Silicon,and I want to simulate that in Matlab for extract the PL signal. (I'm new in...

17 January 2019 3,348 4 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

Best protocol for immunogold using araldite resin ?

Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...

14 February 2021 4,959 1 View

How to improve the adhesion of epoxy coating on steel substrate? Which factor affects?

I am facing a problem regarding a de-adhesion problem with the coating. A brief description of my methodology is given below. I am using mild or Low Carbon steel as a substrate and I polish the...

14 February 2021 2,167 4 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

FLAG purification, elution doesn't have protein?

I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...

02 February 2021 3,860 1 View

How long can synthesized peptides using SPPS be stored? Storage Conditions?

I have been synthesizing peptides on resin beads. I am wondering what best storage method is there to store the beads after synthesis before cleaving. And how long can they be stored in that...

02 February 2021 5,636 5 View

The peptide synthesized with CTC resin have mass more (+68 Da) than the desired mass of the synthesized peptide. What could be the reason?

I am synthesizing 7 amino acid long peptide sequence with CTC resin, 50oC coupling for 10 min. On cleavage (TFA/TIPS/Water 95:2.5:2.5) I got peptide with good yield. However, It has 68 Da more...

30 January 2021 8,483 3 View

What are the governing equations for "Impact Classifier Mill" in Powder Coating Industry?

In Powder Coating Industry, Is there any equation that relates physical properties of chips (granules of thermosetting polymer, i.e, extrudate that has been cooled and crushed) such as dimensions...

26 January 2021 1,077 3 View