How to insert a simply supported boundary condition without in-plane fixation in ANSYS (Transient sructural)?

Dear all, I have a question regarding imposing a boundary condition in ANSYS (Transient Structural). The problem is a slamming of a composite plate in water (FSI problem). The geometry of the...

27 October 2020 640 3 View

Gelatin nano particles, obtained by two-step desolvation, do not re-disperse in water. What is the problem?

Hello I have synthesized gelatin type A nano-particles by two-step desolvation. After 4 days of freeze-drying, the powder was kept in the freezer (-80 C). Today I tried to redisperse the...

18 February 2020 9,494 10 View

Recombinant protein production in insect cells using plasmid-based system?

Is it possible to produce a Flag-tagged catalytic domain of a murine enzyme in insect cells using a plasmid-based system? does it affect the functionality of the final enzyme?

29 November 2019 7,504 3 View

The small dimension of gelatin nano gel. what is the problem?

Hi I am trying to Synthesis gelatin (type A) nano particles by two-step desolvation method. By DLS, the dimension of gelatin particles are less than 50 nm. I am not sure what is wrong with my...

23 July 2019 964 3 View

In MTT or Alamar Blu test for cytotoxicity assay by using UV spectrophotometery, biologists use absorbance even more than 1. Is it correct?

In MTT or Alamar Blu test for cytotoxicity assay by using UV spectrophotometery, I saw many biologist researchers that use absorbance even more than 1 for their calculations. Is it correct? As I...

10 March 2019 4,442 3 View

To make flexible polyurethane foam, necessary to observe the mixing process?

20 June 2016 8,595 7 View

How much GAGs (glycosaminoglycans) is expected of MSCs in their ECM?

Hello, Does anyone know how much GAGs I can collect if I decellularize MSCs in their stemness state and can I stain this GAGs with Alcian Blue? Thanks

23 January 2016 2,565 4 View

What is the integrin binding site for Vitronectin?

As we know vitronectin and some other proteins can bind to different types of integrin. I want to know about the binding site in Integrin which the vitronectin with a RGD-sequence can interact...

30 January 2015 5,378 5 View

How can I extract nodal values of displacement, coordinates and time of each material point at every increment of time in ABAQUS/EXPLICIT?

How can I extract " nodal values of displacement, coordinates and time of each material point at every increment of time" in ABAQUS/EXPLICIT?

30 January 2015 2,597 3 View

Treatments have obvious difference, but ANOVA doesn't show significant difference?

Hello everyone. I attempted one way ANOVA for data set of an experiment with one factor (hydrogel treatment) at 5 levels (5 concentrations). Data showed normal distribution and variance...

26 November 2014 3,450 13 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View