Any suggestion to grow single cell clones of MDA-MB 468 breast cancer cell line after transfection for CRISPR/Cas9 mediated knockout of specific gene?

After transfection with CRISPR plasmids, puromycin selection and single seeding I hardly can see any surviving cells or they die soon in max 2 weeks. I tried gelatine coating, flat or round...

19 January 2018 5,763 5 View