I’m going to inject MC4-L2 cell lines into Balb/c mice. How many cell lines do you suggest for injection into one mouse ? One million or more (2-4 millions)? Which ones could be have the best and fastest result? What type of needle (21g/2ml/3ml) could be better and safer for secure injection (MC4-L2 viability after injection)? Would you have more advice? Please leave a comment. I really appreciate the help.
I study myeloma animal model.
I used NRG mouse and needle type is 26-1/2 syringe.
injection way is I.V. 106 cells at once.(cell line)
I use Balb/c nude mouse and needle type is 26G syringe, injected the MDA-MB231 cell line into the brest (sc) at a number of 10 6 and the model will succe in more than a week!
Thanks.
Why did you use 26 G and 26-1/2 syringe? Would you please tell me what's their advantage compared to others?
I have studied in vitro models of glioma, colorectal and osteossarcoma. All tumors were detected macroscopically when we seeded 4X10E-6 cells. Colon in 3 days, osteossarcoma in 7 days and glioma in 2 weeks.
The little syringe allows easier implantation - number closer to needed - than the bigger, due to the air in needle. Attention!
Hello Denise,
Thank you for your accurate suggestion. Considering your suggestion, Insulin Syringe is better than others. Isn't it?
Hello Delphan
I don't know the size of your cell! In general, 26G and 27G are the most used to xenograft.
These spindle cells are approximately 12-17 million in a T75 flask with 90% confluence.
I had used insulin syringe to implant the tumor cells, Although it is well to implant, the pinhole is too small that it may cause cell damage, so it will influence the mission success rate!
Dear all
Thank you for all of your great recommendation . Nevertheless, please leave a comment if you have any recommend.
An insulin syrunge is good to our use. Take care about the air in needle, only this. Mantain only cells in needle by an unique and stronge movimment to cranial-caudal direction; the air goes to the top of syringe. After that, inject the cells.