How long would be the ideal overhang length when making ligation-independent cloning vectors?

Jeremy Martin Torres @Jeremy-Martin-Torres

17 July 2014 0 4K Report

I'm planning to make my own vector for LIC and the vector I designed had an 18–base overhang on one strand and a 6-base overhang on the other. Do you think these lengths will be enough for ligation-independent cloning?

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