Hello,
I would like to know how long the human primary macrophages (isolated from PCMB and differentiated) survive in culture?
Thank you very much,
Roberto
I was able to follow macrophages for 12 days after PBMC plating if no growth factor was added and also currently have them for 1 months with GM-CSF stimulation (30 ng/ml). M-CSF may keep them alive for a long time as well.
Hi Roberto,
In my experience they usually last about 3 days after the 7 days of differentiation.
Cheers
Daniel
I was able to follow macrophages for 12 days after PBMC plating if no growth factor was added and also currently have them for 1 months with GM-CSF stimulation (30 ng/ml). M-CSF may keep them alive for a long time as well.
I usually use them from day 7, but once differentiated, they do remain alive and healthy-looking for at least a month in RPMI with 10% FCS (at 37C). How healthy they actually are and how they will respond to your treatments at this point however I don't know (after this long, I find the medium remains stable and doesn't yellow at all, suggesting they are much less metabolically active). Also, I would have doubts as to how they would still resemble macrophages in vivo once cultured for this long.
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