Theoretical computer virology ? is still alive ?

HI Everyone! I am asking to know by you, expert in cybersecurity and mathematics, if Computer virology (the one of Cohen and Andler) is still an active field of research. That research made in...

08 February 2021 7,613 6 View

In opensees is the ReinforcingSteel material sensitive to units of measure?

20 January 2021 7,043 2 View

How do I organize items to repeated surveys for quantitative and qualitative analysis?

Hello fellow researchers! Newbie here :) I created a survey with open-ended and Likert-type questions. The survey was administered nine times to the same people using google forms. I plan to...

01 January 2021 3,921 3 View

Is there a better library or function for plotting CFA with many observed variables in R?

Hello! I'm kind of new in using R for doing EFA, CFA, and SEM. I just followed a guide to perform CFA on my data to see how my context fits the ITC-SOPI model in a VR photo-based tour. I used...

23 December 2020 1,679 2 View

Best way to validate CRISPR-Cas9 editing efficiency?

I'm trying to obtain genetic KO in my cell line of interest using Lentiviral-mediated CRISPR-Cas9 delivery system. I would like to test more than one unpubblished sgRNA targetting a couple of...

10 August 2020 1,324 4 View

How to convert .txt into plink files (ped/map or bed/bim/fam)?

I have a huge oncoarray_genotype_XXXX.txt file with 11.7 GB size. I would like to convert this file into adequate files for analyzing in plink software. However, I could not find how to conduct...

08 July 2020 9,302 2 View

Fluorescence labeled antibody?

Hi all, I have a small question for you. Is there any fluorescent labeled antibody available which emits the fluorescence only if bound to substrate? So the unlabeled version does not emit any...

19 March 2020 5,715 2 View

How to evaporate Methyl Acetate, and purge added Ethanol?

To use an inhibitor, prepared and sold in a methyl acetate solution, I need to evaporate the aforementioned solvent and exchanging to ethanol or DMSO. I will do that with a gentle nitrogen stream....

01 March 2020 6,947 3 View

SPSS, data with 5 replicates. How to generate the variables-data sheet?

I am trying to analyze some results obtained from lipidomics. The experimental setup was as follows: 5 replicates, 3 conditions (1 control, 2 treatments). I have thus 5 numerical values per each...

08 January 2020 3,526 3 View

How to solve multicollinearity problem in mixture design?

Due to its own nature mixture design models (mainly with interactions) presents multicollinearity problems, some authors declare that a combination of forward stepwise and step backward stepwise...

05 January 2020 1,136 3 View

What species (or genus even) of crab is this (bivalve using hermit type)?

Two weeks ago I found a peculiar crab in the intertidal zone in SW Florida. when found, the crab was not utilizing a shell. It was similar shaped to porcelain crabs found in our area, but with...

17 January 2021 1,620 4 View

Anyone used "casting traps" to study the blue crab Callinectes sapidus?

Anyone has field experience on eficiency of using this type of casting traps (image attached) versus more "traditional" pots ? If applied to the blue crab, would be optimal. The idea would be to...

11 December 2019 5,478 6 View

What is the best strategy to extract total RNA (Trizol) from crab gills?

Dear all I have tried 1ml Trizol to extract total RNA from crabs gills. The weight of most of my samples is + -0,03 g. We have applied this protocol for hepatopancreas samples and the results...

05 June 2019 6,654 6 View

What is the best strategy to extract total RNA (Trizol) from crab gills?

Dear all I have tried 1ml Trizol to extract total RNA from crabs gills. The weight of most of my samples is + -0,03 g. We have applied this protocol for hepatopancreas samples and the results...

03 June 2019 1,447 8 View

What is the most effective stain/dye for decapods in benthic samples?

I'm sorting benthic suction samples from marsh water interface looking specifically for juvenile crabs 2-16mm. The samples often contain a lot of organic matter, mostly peat, and decomposing marsh...

01 February 2019 4,854 3 View

Who can identificate enigmatic food from stomach of Lyphira perplexa Galil, 2009 crab of South Vietnam (marine fauna)?

My scientific advisor (Burukovsky R.N.) is a famous shrimp researcher and carcinologist. He analyzes food from stomachs of many crustaceans. So... it is an obvious situation when he found an...

11 December 2018 7,797 3 View

Who can identificate enigmatic food from stomach of Lyphira perplexa Galil, 2009 crab of South Vietnam (marine fauna)?

My scientific advisor (Burukovsky R.N.) is a famous shrimp researcher and carcinologist. He analyzes food from stomachs of many crustaceans. So... it is an obvious situation when he found an...

05 December 2018 6,826 3 View

Hormonal studies in hemolymph requires an anticoagulant and antibody?

I'm interested in assessing hormone of mud crabs for my studies. Can anyone share with me if have to use anticoagulant for the hemolymph. Is that fine to run analysis without the anticoagulant?...

09 October 2018 1,479 0 View

Are we underestimating climate change?

I got to the farm today and all I saw was what I assumed to be an early signs of the impacts of climate change. Crabs and scorpions Died from dehydration.

09 October 2018 5,001 1 View

Pls i would like to know the exact primers to detect polyclyclic aromatic hydrocarbons- adduct DNA in Tilapia, crabs and periwinkles ?

Oncology

09 October 2018 9,741 0 View