Hefin Jones

4 Questions 13 Answers 0 Followers

Questions related from Hefin Jones

What are the most reliable phenotypic markers for fibroblasts?

Hi! I don't work with fibroblasts usually but with macrophages and could use some advice. I want to do some transwell co-culture experiments with macrophages and fibroblasts (primary, isolated...

12 May 2015 3,396 4 View

Why would macrophages upregulate CD86 and MHCII in response to IL-4?

Hi all, I'm working with primary, human monocyte-derived macrophages and have been polarizing them in vitro with LPS+IFNy or IL-4 (or medium alone). I've done this many times before in human and...

26 March 2015 541 9 View

Why would cells detach with FURA-2 staining?

Hi, We've been trying to perform Ca2+ flux experiments by dying cells with FURA-2, and then measuring fluorescence using a plate reader in a 96-well format. The protocol has worked beautifully...

26 March 2015 9,337 3 View

How can I calculate the sedimentation time of vesicles?

Hi all, I'm trying to calculate the theoretical time for a vesicle to sediment during centrifugation. I have (I think) all the information, but the numbers I'm getting out don't seem to make...

04 March 2015 7,468 3 View