How long does it take for the exosome to enter the cells?
Does the exosome have an ability to express proteins? or are they just carrying them?
Exosomes are one of the extracellular vesicles (EVs). I am working on EVs, especially EVs derived from plants. Here is a couple of papers that mentioned cellular uptake of plant-derived EVs and protein expression of the target cells.
Lemon: https://pubmed.ncbi.nlm.nih.gov/26098775/
Apple: https://pubmed.ncbi.nlm.nih.gov/30359033/
The uptaken duration can be varied up to your experimental design. For instance, lemon-derived EVs showed the uptake since the 3 hr incubation but apple show at 6 hr. It might be possible to be uptaken faster if you determine it before that time.
In these 2 papers, they show that plant-derived EVs induce the expression of proteins related to cancer cell death and transporter proteins, respectively. But it is the different protein compositions from what they contained for sure.
Hello,
Exosome uptake capability differs depending on the recipient cell type and not on the expression of exosome marker proteins.
In the paper attached, exosome uptake increased with longer incubation time with saturation around 14 hrs. Exosomes are taken up by cells as late as 24hrs.
https://doi.org/10.1155/2014/619829
Exosomes are structures defined as small membrane vesicles eliminated from cells with the information exchange functions between cells, and act as transport vehicles for various biomolecules like proteins, lipids and nucleic acids. So, they are recognized for their role in mediating intercellular communication.
Best.
Exosomes uptake (internalization) depends on the types of cells that are uptaking the exosomes, and the types of exosomes (which cell-derived).
In my experience, exosomes can be uptaken by the cells at the earliest of 4 hr.
Exosomes do not express the proteins. Exosomes carry the proteins from certain cells to other cells.
For the uptake of exosome by most of the cells,6 hours are enough. While increasing the incubation time and giving optimizing dose increase the uptake level as well.
Regards,
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