What software/program you recommend to convert fasta file to Nexus.nxs?

I need to convert my fasta format alignment file to "Nexus.nxs" to be able to use it in Mybeys. What is your suggestion? So far the files I created using Mega x as well as an online database...

09 October 2019 449 1 View

how to export a phylogenetic tree into word document using MEGA X?

I have built a tree of my interest but MEGA X displays an error when saving the image as PDF file! I was wondering how can i save/ copy the tree into a word file. Thanks all

09 October 2019 4,045 5 View

DO you recommend GC-MS/EI or CI?

I am gonna do some analyses / detection on plant secondary metabolites (Terpenes and mostly volatile ones), Was wondering which method might be more beneficial- GC-MS-EI or CI? In most recent...

09 October 2019 2,384 2 View

Problem with trimAL installation?

Is anyone can help in how to install trimAL on windows 10? I am gonna use the software for trimming of my aligned sequences. It seems the software does not get installed in my system! Any idea...

08 September 2019 4,083 3 View

Do I need to carbonize the EM formvar grids to visulize virus like particles?

Dear researchers, I am going to fix my virus like particle sample using negative staining. However, the grids i have are only formvar without carbon coated. Do you have any idea what difference it...

10 November 2018 5,842 7 View

Best method to resuspend and anneal oligose for downstream ligation?

Dear Researchers, I have ordered oligose which should be annealed together for cloning/ ligation experiment. They are 66 in length and stickiy ends using two different restriction enzymes. The MW...

06 July 2018 9,829 4 View

Do I need to design a specific antibody for purification of my small recombinant peptides?

I am fusing small peptides around 10 to 30 a.a into the C-terminus of a larger protein. The question is if I need to design specific antibodies against those small epitopes? I actually have two...

04 May 2018 1,635 2 View

Why my SDS PAGE get stuck in the middle of the gel and the last lanes of the ladder are not seperating properly?

Dear All, I recently have a problem with my SDS-PAGE running. After staining with Brilliant blue and de-staining, I see that the proteins have not been run until the end of the gel. I increased...

04 May 2018 4,267 13 View

Recommending a protocol for VLPs isolation from e.coli?

Hi all, Iam expresing a virus capsid protein in e.coli. I need an optimised protocol for isolation virus like particles from e.coli cells. Using ultarcentrifugation I have a protocol but usually...

04 May 2018 8,178 2 View

Does anyone know of a plant based-vaccine against HAV?

Hi all, I am looking for some papers/ publications where a Hepatitis A vaccine has been tried or made in any plant. To my knowledge, There are not that much study on plant based vaccine against...

01 February 2018 2,556 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View