Recommending a protocol for VLPs isolation from e.coli?

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What software/program you recommend to convert fasta file to Nexus.nxs?

I need to convert my fasta format alignment file to "Nexus.nxs" to be able to use it in Mybeys. What is your suggestion? So far the files I created using Mega x as well as an online database...

09 October 2019 449 1 View

how to export a phylogenetic tree into word document using MEGA X?

I have built a tree of my interest but MEGA X displays an error when saving the image as PDF file! I was wondering how can i save/ copy the tree into a word file. Thanks all

09 October 2019 4,045 5 View

DO you recommend GC-MS/EI or CI?

I am gonna do some analyses / detection on plant secondary metabolites (Terpenes and mostly volatile ones), Was wondering which method might be more beneficial- GC-MS-EI or CI? In most recent...

09 October 2019 2,384 2 View

Problem with trimAL installation?

Is anyone can help in how to install trimAL on windows 10? I am gonna use the software for trimming of my aligned sequences. It seems the software does not get installed in my system! Any idea...

08 September 2019 4,083 3 View

Do I need to carbonize the EM formvar grids to visulize virus like particles?

Dear researchers, I am going to fix my virus like particle sample using negative staining. However, the grids i have are only formvar without carbon coated. Do you have any idea what difference it...

10 November 2018 5,842 7 View

Best method to resuspend and anneal oligose for downstream ligation?

Dear Researchers, I have ordered oligose which should be annealed together for cloning/ ligation experiment. They are 66 in length and stickiy ends using two different restriction enzymes. The MW...

06 July 2018 9,829 4 View

Do I need to design a specific antibody for purification of my small recombinant peptides?

I am fusing small peptides around 10 to 30 a.a into the C-terminus of a larger protein. The question is if I need to design specific antibodies against those small epitopes? I actually have two...

04 May 2018 1,635 2 View

Why my SDS PAGE get stuck in the middle of the gel and the last lanes of the ladder are not seperating properly?

Dear All, I recently have a problem with my SDS-PAGE running. After staining with Brilliant blue and de-staining, I see that the proteins have not been run until the end of the gel. I increased...

04 May 2018 4,267 13 View

Does anyone know of a plant based-vaccine against HAV?

Hi all, I am looking for some papers/ publications where a Hepatitis A vaccine has been tried or made in any plant. To my knowledge, There are not that much study on plant based vaccine against...

01 February 2018 2,556 1 View

How pET29 expresses the HIS-tag? Is it possible that a truncated recobminant protein or a his-tag is expressed after IPTG induction?

I have cloned my gene of interest into pET29c, and sequencing verified it too. I used a c-terminus his-tag in my insert. However, when i do induction (1mM IPTG, Over night at 28C), I see a band...

31 December 2017 7,731 9 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View