30 October 2017 1 2K Report

I add protease inhibitors to my lysis buffer that I resuspend the cell pellet in. However, during the chromatography purification process, I am performing dialysis and changing buffers several times. Do I need to add protease inhibitors so all of these buffers and dialysis steps? All of these buffers contain EDTA and EGTA and are pH 8.

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