How long can I kept tissue at -80 C ?

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Gregory Guerra Bieberach

hello Nadia!

Freeze and thaw cycles will affect the RNA integrity and original proportions.

There are chemical treatments that work better than storage in the market available, they will reinforce RNA in a way that it would not be affected by storage or isolation methods.

Nadia S. Mahmoud

Thanks for replying. I know but what if keep it for example for 2 months at these conditions without repeated thawing and freezing would it affect RNA?

Hassan Nima

This link is useful

Article Long-Term Storage of Endocrine Tissues at −80°C Does Not Adv...

Regards

Khalid Al-Salhie

I think you can it for 2-3 months.