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Questions related from Gregory Guerra Bieberach
Hello, i'm looking for a good server for download satelital images for Master thesis (crop production) , ESA-SENTINEL has 10m resolution but i'm afraid I need
19 August 2021 5,767 1 View
Dear all, below I have attached a picture of the wasp in question, has an average of 1 cm of lenght and 0,4 cm in width, behaviour was not hostile, no nest was recognizible or found. thank you...
09 January 2019 10,044 4 View
I would like to know about the theory behind this method to achieve an 100% oxygen saturation standard, According to my manuals: "For the standard 100% Oxygen saturation, fill a cup with 4 mm of...
13 November 2017 9,093 2 View
hello everyone, i hope you can help me about this troublesome quest: -I will study 2 conditions with 3 biological replicates (3 samples in control vs 3 samples in treatment) (6 samples in...
11 August 2016 7,702 3 View
Greetings to you all: -I only got to test 4 ref. genes -In spite of that, I obtain in geNorm M values lower than 0.5 and the best couple got a 0.217 M value. -Then, I chosed 3 ref. genes since...
06 August 2016 6,996 1 View
First, I am thinking about extracting RNA from the same number of bacteria from 2 differents conditions. Secondly, there would be a quantification and normalization step of RNA ammounts? In...
11 January 2016 3,957 2 View
in the same culture, the strain (bacilli) shows variable size and even shape, some of them are streptobacilli, I wonder if is this common? this strain have been isolated from plate culture...
02 December 2015 2,736 4 View
I've been obtaining mixed results regarding to duplication time in bacterial kinetics of some strain I'm studying. Duplication times vary from 54 to 64 hrs How much variation should be allowed?
23 November 2015 5,388 5 View
During the phase of exponential growth in my bacteria, I have managed to stablish a exponential regression coefficient of 0.96. Is this enough to claim that this is in fact and exponential...
15 October 2015 2,970 3 View
Im having trouble getting the right efficency for my primers doing the standart procedure of serial dilutions. But I soon as I correct the dilutions using a quantifyer like Qubit reagent for...
30 May 2015 1,413 5 View
can anybody recommend me some softwares for do so? Im running reaI time reIative ct/ct anaIysis in 7500 step one pIus.
05 February 2015 817 1 View
Is it possible to perform RT PCR assays were targets have different TH? Especially reference genes, doing some reference genes at one reaction with some targets and the rest with the other sampIes?
27 January 2015 7,099 2 View
Is a 3´ end heterodimer of Delta G: -1.6 kcal/mole with 2 base pairs of bridge going to affect the real-time PCR primer efficiency? I would like to know of someone who has used primers with these...
26 November 2014 2,787 3 View
