Gregory Guerra Bieberach

9 Questions 23 Answers 0 Followers

Questions related from Gregory Guerra Bieberach

Best resolution, lower priced satelital image service available?

Hello, i'm looking for a good server for download satelital images for Master thesis (crop production) , ESA-SENTINEL has 10m resolution but i'm afraid I need

19 August 2021 5,715 1 View

Why is 100% Oxygen Saturation standard measured in a water column close to the probe?

I would like to know about the theory behind this method to achieve an 100% oxygen saturation standard, According to my manuals: "For the standard 100% Oxygen saturation, fill a cup with 4 mm of...

13 November 2017 9,044 2 View

Troubles regarding plate design for qPCR experiment...help?

hello everyone, i hope you can help me about this troublesome quest: -I will study 2 conditions with 3 biological replicates (3 samples in control vs 3 samples in treatment) (6 samples in...

11 August 2016 7,649 3 View

Are these reference genes (relative quantification qPCR) suitable according to results?

Greetings to you all: -I only got to test 4 ref. genes -In spite of that, I obtain in geNorm M values lower than 0.5 and the best couple got a 0.217 M value. -Then, I chosed 3 ref. genes since...

06 August 2016 6,945 1 View

Duplication time in bacteria: How much variation should be allowed?

I've been obtaining mixed results regarding to duplication time in bacterial kinetics of some strain I'm studying. Duplication times vary from 54 to 64 hrs How much variation should be allowed?

23 November 2015 5,341 5 View

Is an exponential regression coefficient of 0.96 enough for Bacterial Growth?

During the phase of exponential growth in my bacteria, I have managed to stablish a exponential regression coefficient of 0.96. Is this enough to claim that this is in fact and exponential...

15 October 2015 2,919 3 View

Can one "correct" dilutions prepared for qPCR standard curve?

Im having trouble getting the right efficency for my primers doing the standart procedure of serial dilutions. But I soon as I correct the dilutions using a quantifyer like Qubit reagent for...

30 May 2015 1,362 5 View

Is it possibLe to perform RT-PCR with different Hybridization Temps?

Is it possible to perform RT PCR assays were targets have different TH? Especially reference genes, doing some reference genes at one reaction with some targets and the rest with the other sampIes?

27 January 2015 7,044 2 View

Is a 3´ heterodimer >- 2 kcal/mol going to affect primer efficiency in real-time PCR?

Is a 3´ end heterodimer of Delta G: -1.6 kcal/mole with 2 base pairs of bridge going to affect the real-time PCR primer efficiency? I would like to know of someone who has used primers with these...

26 November 2014 2,733 3 View