QPCR troubleshooting - what could cause an increasing ROX signal?

Hi everyone, I'm looking for some help troubleshooting a recent qPCR issue I've encountered. I am using fluorescent Taqman probes and a qPCR master mix that includes ROX as a passive reference....

08 February 2021 419 3 View

BrdU staining issue?

Hi everyone. I bumped into the difficulty that I couldn't get any signals from my brain section in the DG region of hippocampus. However, I have standardized the BrdU protocol in my brain...

13 January 2021 9,449 0 View

Effective number of species and Wilcoxon Signed Rank Test for diversity between 2 ecosystems: is this accurate?

Hi! So I'm currently studying the diversity difference between 2 ecosystems. I ran a Shannon-Wiener test on site 1 and site 2 and found the indices of 1.525 and 2.165 , respectively. I have done...

22 November 2020 6,798 1 View

How to reproduce the sub-grade of pavements without using soil?

Dear colleagues, In order to evaluate the performance of concrete slabs-on-grade, the sub-grade should also be considered in the testing setup. Using soil or sand to reproduce the sub-grade is a...

20 November 2020 7,921 1 View

How to determination of available silicon in fertilizer by UV-Vis?

Hello everyone! I have a problem with when the determination of available silicon in fertilizer by UV-Vis. I do that following method A 5-Day Method for Determination of Soluble Silicon...

10 November 2020 1,370 10 View

What is the problem with my 10um Nissl stained cell?

Please see the attachment. In my case, I tried a lot of time on the Nissl staining on 10um cryosection. I am not sure which step goes wrong? I make sure my anti-rolling glass is intact. seem like...

07 November 2020 9,568 3 View

Where and how do I specify frequency in the route section of frequency-dependent calculation (Gaussian using Linux)?

Hello everyone. I would like to compute the dynamic hyperpolarizability value of a molecule using Gaussian on Linux OS. I have read that the essential keywords to include for such request are the...

07 November 2020 5,620 4 View

What type of regression should I be using?

I am planning to conduct a study with the following variables: A - independent variable B - intervening variable C - dependent variable D - moderating variable between B and C The framework...

22 October 2020 5,324 3 View

Not sure what does 3mg/kg mean in AD STZ injection?

What I searched the paper online they mentioned do 3mg/kg ICV on mice On Day 1 and Day3. Doese it mean total 3mg/kg for 2 days, or 1.5mg/kg for day1 and another 1.5mg/kg for day3? Help help to...

19 October 2020 8,687 3 View

How do I calculate theoretical fluorescence spectra (TDDFT) in Gaussian 09W in selected solvent?

Good day everyone. I am new to computational chemistry and would like to integrate some part of it in my fluorescence study. I would like to investigate the theoretical fluorescence spectra using...

18 September 2020 6,742 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View