Hayley Chan

3 Questions 4 Answers 0 Followers

Questions related from Hayley Chan

How does one prepare BSA for ChIP assay?

I am preparing a BSA solution for ChIP assays, the BSA solution will be used as a blocker for ChIP antibody binding beads. Should I prepare the BSA in Milli-Q water or PBS or TBS or is there any...

22 October 2014 6,352 3 View

How long can I incubate C3H10T1/2 cells (or 3T3-L1 cells) with cell permeable PKA inhibitor 14-22 amide?

And should I incubate them at 37C or room temperature? If at RT, should it be in the dark? I am differentiating the cells into adipocytes, using the MDI protocol (IBMX, dexamethasone and insulin)...

01 October 2014 8,982 2 View

Is there a standard assay for testing whether a commercial PKA inhibitor works or not?

PKA inhibitor

23 September 2014 9,278 2 View