The question is what did you want? The Link Saikat posted is good to compare sequence data like ATGATCTATCCATAGCTA. You can use this for compare wild-typ and mutations for example but you have only the nucleotid code. What is also very important is to look to the interferogram like you see on this picture: http://upload.wikimedia.org/wikipedia/commons/5/5c/DNA_sequencing_interferogram.gif because for example if you have human genomic DNA for analysis you sometimes only here see heterozygous mutations and not in the fasta file from the sequencing. So maybe the program Chromas will also help you.
Hi Reza. In order to get significant answers, one must ask a well defined question. It is impossible to guess what you want from such a short and vague question. It is thus impossible to help you. Please take the time to explain, with enough details what type of data you have (technology, read length, number of reads...), what you wish to accomplish with this data (population genetics, SNP discovery, gene annotation...) and what are the steps you think are needed. Then ask a specific question about what software would be best to solve your specific and well defined problem. Lots of people will be ready to help when you make the effort of explaining you problem properly. Cheers
you can use BLAST tool available in the NCBI website. or, if you want to precisely want to compare with the original sequence, use DNA Baser which is a free software for certain hours. good one. or other softwars like vector NTI, sequencher, or codoncode must be useful. But, you need to purchase them.
I agree with Eric Normandeau this question really isn't specific enough.
But sometimes vague questions yield the best answers as provided above.
So to add to this collection, you can BLAST your sequence as per Chelladurai Rathnasingh's really good suggestion.
However (I am guessing) if you submitted a sample for sequencing and just got it back and want to see it on a chromatogram viewer. I would suggest you use (the free) finch TV. Its easy to use and install. I believe (its been a couple of years) all you need is a .txt file to input the data.
you should know yours gene and its sequence( from www.ncbi.org) reach in gene to get sequence in fasta format. then alignment the getting seq. with origin seq.( from ncbi) by using BioEdit or lasargene( expensive).
Well, depending what your research is about I sugest you to BLAST the sequence of the gene you are trying to study and compare with the sequences from ncbi. But first you have to obtain the sequence of your gene in fasta format and analyse the quality of the sequence that you obtained.I also use the BioEdit software to do the alignment of the sequences and analyse these sequences to uderstand it better. Good luck!
In case of overlapped peaks, if the correct one is short peak with a wrong overlapping long peak, is it allowed for me to edit the sequence by adding the short right one instead of the wrong long one?