Well trending times and use of dueteriated compounds had solved many problems including ion suppression effects, well everytime we need not go for also there are number of bile acids you havent sepcified particular, hence if you can have any other structurally analog similar kind then it can be used as ISTD internal standard. This can be used if you had found a rugged reproducible ion suppression enhancement free method for your need of bile acid.
This depends a lot on your specific needs. As Rajen Shankallpura noted, dueterated internal standards are good. But these can be expensive and not always available. Nonetheless, if you're looking at only a few analytes, if you can use deuterated or otherwise labeled standards, this would be great. This means that - for each bile acid that you're studying - you have the same structure but with isiotopic labels. (With dueterated standards,, though, you need to be sure that there's no H-D exchange under the conditions of the experiments.)
If you're analyzing bile-acids in general, then it's not so demanding; you need something that behaves similarly to your analytes both with respect to chromatography and mass spectrometry.
Also, depending on your systems, you may want to look at secondary metabolites, e.g., glucuronides.
In general, standards should match, as best as possible, the behavior of the analytes with no chance that they'd be there otherwise.
thank you for your responses. What about the role of Pka and PH of a specific kind of bile acid (e.g CA 3-5 and Pka5-6.5) to select the internal standard. Are they important? Also, is it possible to have a general standard for diffrent types of bile acids?
Thanks for your comments.
From my laboratory, a number of deuterated internal standards is available, also for a few bile acids. [email protected]
We analyze bile acids following this paper (see below). Its part of our routine analysis.
Thanks Dear Andre, Have you changed anything in your method based on this paper or you just followed the method. As I have read that you mentioned.
thanks
- Badges
- Science topic
- Similar topics
- Chemistry
- Biochemistry