How can we detect over expression of a gene?

Sometimes some gene gets over expressed in organism, so which molecular technique can help us to detect the over expression of that gene?

06 July 2019 9,406 5 View

How many bands will I find if I run human extracted DNA through agarose gel electrophoresis?

Human bears separate 46 chromosomes. If we extract DNA from human sample then how many band will be visible on gel?

08 September 2018 6,350 5 View

Do pH maintaining chemicals interfere in DNA extraction?

We use EDTA, Tris-HCl and some other chemicals, whose pH remain almost 8.0. To balance pH 8.0 we use NaOH. Now I would like to know that; have these unwanted NaOH, HCl or Acetic Acid (if...

04 May 2018 5,684 1 View

Which is the solution used for pH meter sensor?

I need to know that which solution is used to keep the pH meter sensor wet. Some people say that KCl is used but which concentration and pH are better?

31 December 2017 5,670 3 View

How can we measure the band size that greater than 10Kb?

07 August 2017 3,413 2 View

Why do bacteria lose their plamids at normal condition?

Plasmids are extra chromosomal DNA of bacteria, but they have numerous functions for the survival and living of bacteria. It is claimed that bacteria lose their plasmids when they live without...

07 August 2017 3,918 9 View

Is it mandatory to culture Bacteria in an antibiotic rich medium before plasmid extraction?

It is claimed that bacteria lose their plasmids unless they are cultured in a antibiotic maintained medium. As a result a negative consequence will arise after plasmid DNA extraction. How will you...

07 August 2017 1,637 6 View

Where are the nod and nif genes of Rhizobia located?

07 August 2017 9,745 2 View

Is it possible to culture coffee plant in vitro?

Is anywhere it being tissue culture of Coffee plant in Bangladesh?

04 May 2017 4,613 2 View

What is selfish Rhizobia?

Somebody claims that there some selfish rhizobia which take benefits from the host but do not induced nodulation at the host. I did not get enough evidence about this. Could anybody help me by...

07 August 2016 3,089 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View