Using bioinformatics how i can design a new drug or protein, can any one help me?
Sometimes some gene gets over expressed in organism, so which molecular technique can help us to detect the over expression of that gene?
06 July 2019 9,494 5 View
Human bears separate 46 chromosomes. If we extract DNA from human sample then how many band will be visible on gel?
08 September 2018 6,428 5 View
We use EDTA, Tris-HCl and some other chemicals, whose pH remain almost 8.0. To balance pH 8.0 we use NaOH. Now I would like to know that; have these unwanted NaOH, HCl or Acetic Acid (if...
04 May 2018 5,763 1 View
I need to know that which solution is used to keep the pH meter sensor wet. Some people say that KCl is used but which concentration and pH are better?
31 December 2017 5,743 3 View
We generally use 100bp and 1Kb ladders to measure the bands size of extracted bacterial DNA but what should we use to measure the bands, are greater than 10Kb?
07 August 2017 3,486 2 View
Plasmids are extra chromosomal DNA of bacteria, but they have numerous functions for the survival and living of bacteria. It is claimed that bacteria lose their plasmids when they live without...
07 August 2017 3,987 9 View
It is claimed that bacteria lose their plasmids unless they are cultured in a antibiotic maintained medium. As a result a negative consequence will arise after plasmid DNA extraction. How will you...
07 August 2017 1,712 6 View
The nitrogen fixing rhizobia have different nod and nif genes but where is their location? Are the genes located in plasmids or in the chromosome of the bacteria?
07 August 2017 9,822 2 View
Is anywhere it being tissue culture of Coffee plant in Bangladesh?
04 May 2017 4,686 2 View
Somebody claims that there some selfish rhizobia which take benefits from the host but do not induced nodulation at the host. I did not get enough evidence about this. Could anybody help me by...
07 August 2016 3,171 6 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed
30 July 2024 1,061 5 View