We generally use 100bp and 1Kb ladders to measure the bands size of extracted bacterial DNA but what should we use to measure the bands, are greater than 10Kb?
Lambda DNA/HindIII marker is used for the purpose. It contains bands from 125 bp to 23 kb.
You can use PFGE or its variations, if you want to measure DNA longer than 20 Kbp, it can be used to measure DNA upto 5 Mbp .
Sometimes some gene gets over expressed in organism, so which molecular technique can help us to detect the over expression of that gene?
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Is anywhere it being tissue culture of Coffee plant in Bangladesh?
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