How can we measure the band size that greater than 10Kb?

More Syed Raju Ali's questions See All

How can we detect over expression of a gene?

Sometimes some gene gets over expressed in organism, so which molecular technique can help us to detect the over expression of that gene?

06 July 2019 9,494 5 View

How many bands will I find if I run human extracted DNA through agarose gel electrophoresis?

Human bears separate 46 chromosomes. If we extract DNA from human sample then how many band will be visible on gel?

08 September 2018 6,428 5 View

Do pH maintaining chemicals interfere in DNA extraction?

We use EDTA, Tris-HCl and some other chemicals, whose pH remain almost 8.0. To balance pH 8.0 we use NaOH. Now I would like to know that; have these unwanted NaOH, HCl or Acetic Acid (if...

04 May 2018 5,763 1 View

Which is the solution used for pH meter sensor?

I need to know that which solution is used to keep the pH meter sensor wet. Some people say that KCl is used but which concentration and pH are better?

31 December 2017 5,743 3 View

Why do bacteria lose their plamids at normal condition?

Plasmids are extra chromosomal DNA of bacteria, but they have numerous functions for the survival and living of bacteria. It is claimed that bacteria lose their plasmids when they live without...

07 August 2017 3,987 9 View

Is it mandatory to culture Bacteria in an antibiotic rich medium before plasmid extraction?

It is claimed that bacteria lose their plasmids unless they are cultured in a antibiotic maintained medium. As a result a negative consequence will arise after plasmid DNA extraction. How will you...

07 August 2017 1,712 6 View

Where are the nod and nif genes of Rhizobia located?

The nitrogen fixing rhizobia have different nod and nif genes but where is their location? Are the genes located in plasmids or in the chromosome of the bacteria?

07 August 2017 9,822 2 View

Is it possible to culture coffee plant in vitro?

Is anywhere it being tissue culture of Coffee plant in Bangladesh?

04 May 2017 4,686 2 View

What is selfish Rhizobia?

Somebody claims that there some selfish rhizobia which take benefits from the host but do not induced nodulation at the host. I did not get enough evidence about this. Could anybody help me by...

07 August 2016 3,171 6 View

What are the morphological characteristics of fast growing Rhizobium japonicum?

I need to know the morphological feature of fast growing Rhizobium spp. For example, time required to form single colony, colony size, color, salt tolerance, BTB test feature, congured test etc.

31 December 2015 3,493 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View