What is the difference between anionic and cationic redox probes?

I have done Kinetics study of two different probes namely Fe(CN)63-/4- and Ru(boy)2+ on glassy carbon electrode. I kept the potential between -0.2 to 0.6 and 0.8 to 1.2 respectively. In FeCN when...

25 April 2020 3,364 10 View

What are the high yielding methods of extraction of phycocyanin from dry mass of Anthirospira plantesis?

extraction techniques

31 January 2020 7,268 1 View

How to find the dihedral angle of particular residue over the period of simulation time?

Dear Friends, I would like to perform the dihedral angle calculation of particular residue over the period of simulation time. Could you please help me out in creating the ndx file using make_ndx...

19 March 2018 2,284 3 View

How to find the actual coordinate form the normailzed image value?

Hi, For example, I have a 500*500 size of image, but I normalized the range of [0 1], then how to find the image actual coordinate value from the normalized image value

08 February 2018 2,348 4 View

Can any one suggest the reference book for Data Analytics?

Dear Researcher (s) Can any one suggest the reference book for Data Analytics?

11 December 2017 6,158 2 View

How to perform the PIEDA calculation for the receptor-ligand residues using FMO ?

Dear All, Im trying to compute the PIEDA for the receptor-ligand residues using the FMO calculation. Could you help me in providing the steps to create the input put file for running the...

10 November 2017 144 2 View

How to calculate the host-guest binding affinity using quantum mechanics

16 June 2017 1,904 3 View

What are the tools are available for pattern recognition?

Hi.. I am working on biometric with MATLAB. It is really hard for me. So what are the tools are available for pattern recognition?

10 October 2016 6,507 9 View

Is it possible to make Transparent solar panels using perovskite? If No why?

Transparent solar panels

25 July 2016 6,953 6 View

Can we able to measure the thickness of the thin film sample using X ray diffraction?

Thin film, Thickness measurement, Elipsometry, XRD

10 July 2016 9,256 9 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View