I intend to utilize a 2-inch plant, since it takes about a year for a plant to grow from the root. So, I must remove the native microorganisms (that is already present in the plants and their roots without killing the plants). I am planning to use the plant for understanding PGPR.
on a laboratory scale, I think it is possible to kill microorganisms by washing with alcohol. or with other disinfectants. Appropriate poisons can be used on a large scale. it depends on what the purpose of removing the microorganism is.
Good luck.
Nharmadhaa Manickam 1) The problem is that there are many microorganisms inside the plants too. Only plants grown in vitro from meristema can be accepted as free from such microbes. 2) Any antiseptic will affect plant tissues mush more than microbes. 3) Microbiota characterisation before PGPR application is the possible way of working with non-sterile plants.
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