extraction techniques
Interesting question
I intend to utilize a 2-inch plant, since it takes about a year for a plant to grow from the root. So, I must remove the native microorganisms (that is already present in the plants and their...
28 April 2024 3,055 2 View
Hi all, I have used the standard MKHS composition to make this buffer. The initial pH of the solution was 7.7. I adjusted the pH to 7.3 with 1N HCL and bubbled with carbogen (95% O2 and 5% CO2)...
13 September 2023 3,052 3 View
In a thick cylindrical pressure vessel, what are all the types of stresses acting on them? and tell that magnitude of various stresses acting on thick cylindrical pressure vessel in decreasing...
08 January 2021 7,549 0 View
Nuclei isolation from Pancreas? All the nuclei that I have isolated look good but they are all clumped together in some sort of gelatinous matrix? Does anybody have any inputs to improve the...
03 April 2020 8,187 2 View
Hello, Had anyone used antibodies from Signalway, Sabbiotech, USA for western blotting?. If so, kindly gives your comments about the quality of primary antibodies.
29 July 2018 2,190 1 View
Hi, For example, I have a 500*500 size of image, but I normalized the range of [0 1], then how to find the image actual coordinate value from the normalized image value
08 February 2018 2,525 4 View
i need some clarifications for dye extraction method. i am working in TiO2 dye sensitized solar cells.
09 January 2018 7,549 3 View
Dear Researcher (s) Can any one suggest the reference book for Data Analytics?
11 December 2017 6,308 2 View
The calculated specific capacitance value from GCD analysis is comparatively low with CV analysis. why? can any one explain?
27 July 2017 7,166 6 View
I have problem on lifetime rawfile, so how to convert in this X and Y axis from lifetime rawfile.
07 June 2017 2,484 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,081 3 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,140 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,402 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,881 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,328 0 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 500 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,827 0 View
EDX analisys
31 July 2024 5,946 2 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,149 0 View
