Hi all,
I have used the standard MKHS composition to make this buffer. The initial pH of the solution was 7.7. I adjusted the pH to 7.3 with 1N HCL and bubbled with carbogen (95% O2 and 5% CO2) for 30 minutes. Later the pH changed to 7.8. If I prolong the carbogen bubbling the pH goes beyond 8.0. We checked the percentage of CO2 in the gas mixture it is 4.98%. But in the solution after gassing the pCO2 goes down, compared to the ungassed MKHS. Where could be the potential problem lies?
Could anyone help to solve this problem?
TIA
Kesavan